bs-6569R [Primary Antibody]
Rabbit  Anti-RLBP1L1  Polyclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Rabbit

Target Protein: RLBP1L1

IR: Immunogen Range:231-330/354

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 157807

Swiss Prot: Q8IUQ0

Source: KLH conjugated synthetic peptide derived from human ICRF/REG1 alpha:231-330/354 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: RLBP1L1 is a protein of unknown function. It has 45% identity with human CRALBP (Cellular retinaldehyde-binding protein) which carries 11-cis-retinol or 11-cis-retinaldehyde as endogenous ligands and may function as a substrate carrier protein that modulates interaction of these retinoids with visual cycle enzymes. RLBP1L1 is expressed in brain, and several human carcinoma cell lines.

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RLBP1L1 Polyclonal Antibody, Unconjugated(bs-6569R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RLBP1L1) Polyclonal Antibody, Unconjugated (bs-6569R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
PRODUCT SPECIFIC PUBLICATIONS