bs-0788R [Primary Antibody]
Rabbit  Anti-intestinal FABP  Polyclonal Antibody
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Host: Rabbit

Target Protein: intestinal FABP

IR: Immunogen Range:45-132/132

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2169

Swiss Prot: P12104

Source: KLH conjugated synthetic peptide derived from IFABP:45-132/132 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Intestinal FABP is an intestinal marker. It is expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips. It is thought to play a role in the intracellular transport of long chain fatty acids and their acyl CoA esters and may be involved in triglyceride rich lipoprotein synthesis.

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse (predicted: Rat,Rabbit,Pig,Chicken,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Intestinal(Mouse) Lysate at 30ug; Primary: Anti-intestinal FABP (bs-0788R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 15 kD Observed band size: 17 kD
Sample: Raji Cell Lysate at 30ug; Primary: Anti-intestinal FABP (bs-0788R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 15 kD Observed band size: 17 kD
Tissue/cell: human colon carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IFABP Polyclonal Antibody, Unconjugated(bs-0788R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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