bs-6073R [Primary Antibody]
Rabbit  Anti-MFSD2A Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: MFSD2A Rabbit pAb

IR: Immunogen Range:331-430/543

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84879

Swiss Prot: Q8NA29

Source: KLH conjugated synthetic peptide derived from human MFSD2A:331-430/543 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: MFSD2 is a 543 amino acid multi-pass membrane protein of the endoplasmic reticulum that is involved in beta-adrenergic signaling during thermogenesis. Existing as three alternatively spliced isoforms, MFSD2 plays a role in G1 regulation and is encoded by a gene that maps to human chromosome 1p34.2. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/test

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with MFSD2A polyclonal antibody, unconjugated (bs-6073R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-MFSD2A Polyclonal Antibody, Unconjugated(bs-6073R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-MFSD2A antibody (bs-6073) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 0.2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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