bs-6506R [Primary Antibody]
Rabbit  Anti-ABI3BP  Polyclonal Antibody
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Host: Rabbit

Target Protein: ABI3BP

IR: Immunogen Range:931-1075/1075

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25890

Swiss Prot: Q7Z7G0

Source: KLH conjugated synthetic peptide derived from human ABI3BP:931-1075/1075 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: ABI3BP (ABI gene family member 3-binding protein) contains 2 fibronectin type-III domains and is thought to interact with ABI3. It is expressed in brain, heart, lung, liver, pancreas, kidney and placenta. ABI3BP expression is dramatically reduced in human lung cancer cell lines and primary lung tumor, while it is predominantly expressed in normal conditions, suggesting that it is involved in prevention of tumorigenesis.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Placenta (Mouse) Lysate at 40 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti- ABI3BP (bs-6506R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 116 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ABI3BP Polyclonal Antibody, Unconjugated(bs-6506R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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