bsm-60706R [Primary Antibody]
Rabbit  Anti-Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit mAb   Antibody
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Host: Rabbit

Target Protein: Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit mAb

IR: Immunogen Range:SD(Ac-K)TI

Clonality:

Isotype: IgG

Entrez Gene: 7277

Swiss Prot: P68366

Source: KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40):SD(Ac-K)TI 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:1000-4000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:25,ICC/IF=1:25

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:6000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 50 kDa Observed MW: 55 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7+serum starvation(14hr), (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr) Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 50 kDa Observed MW: 55 kDa
Tissue: Rat cerebellum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60706R
Tissue: Human cerebrum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60706R
 
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