bs-0938R [Primary Antibody]
Rabbit  Anti-NKG2D Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: NKG2D Rabbit pAb

IR: Immunogen Range:121-219/219

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24934

Swiss Prot: O70215

Source: KLH conjugated synthetic peptide derived from rat NKG2D:121-219/219 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,Flow-Cyt=2μg/Test

Cross Reactive Species: Human,Mouse (predicted: Rat)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NKG2D (bs-0938R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 26 kD
Blank control (blue line): Mouse thymus cells (blue). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:SW480. Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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