bs-1753R [Primary Antibody]
Rabbit  Anti-ECE2 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: ECE2 Rabbit pAb

IR: Immunogen Range:601-700/883

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9718

Swiss Prot: P0DPD6

Source: KLH conjugated synthetic peptide derived from human ECE2:601-700/883 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Endothelin-converting enzyme 2 (ECE2) converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity. Expressed at high levels in central nervous system. Expressed in adrenal glands, ovary and uterus, and at low levels in heart.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-ECE2 (bs-1753R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80-100 kD Observed band size: 80 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ECE2) Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
 
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