bs-2127R [Primary Antibody]
Rabbit  Anti-5HT4 Receptor Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: 5HT4 Receptor Rabbit pAb

IR: Immunogen Range:21-120/388

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3360

Swiss Prot: Q13639

Source: KLH conjugated synthetic peptide derived from human 5-HTR4:21-120/388 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800

Cross Reactive Species: Human,Mouse,Rat (predicted: Cow,Chicken)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-5HT4 Receptor (bs- 2127R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD
Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-5HT4 Receptor (bs-2127R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD
Sample: Lane 1: Stomach (Mouse) Tissue Lysate at 40 ug Lane 2: Cerebrum (Mouse) Tissue Lysate at 40 ug Lane 3: Heart (Mouse) Tissue Lysate at 40 ug Lane 4: Adrenal gland (Mouse) Tissue Lysate at 40 ug Lane 5: Adrenal gland (Rat) Tissue Lysate at 40 ug Primary: Anti-5HT4 Receptor (bs-2127R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (bs-2127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (bs-2127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (bs-2127R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
 
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