bs-11250R [Primary Antibody]
Rabbit  Anti-EEA1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: EEA1 Rabbit pAb

IR: Immunogen Range:1251-1350/1411

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8411

Swiss Prot: Q15075

Source: KLH conjugated synthetic peptide derived from human EEA1:1251-1350/1411 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Early endosomes are cytoplasmic compartments that function in receiving and sorting endocytosed proteins for vesicular transport. EEA1 (early endosome antigen 1) is a peripheral membrane protein that co-localizes with the transferrin receptor and Rab5 on early endosomes. EEA1 contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. EEA1 has sequence homology to several yeast proteins that have been implicated in membrane trafficking, including Vps27, Fab1 and Vac1. Evidence suggests a possible role for EEA1 in mediating the regulatory effects of 3'-phosphoinositides on membrane trafficking.

Size: 200ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test

Cross Reactive Species: Human,Hamster (predicted: Mouse,Rat,Cow,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Tissue/cell: human stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EEA1 Polyclonal Antibody, Unconjugated(bs-11250R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(black line):Hela. Primary Antibody (green line): Rabbit Anti-EEA1 antibody (bs-11250R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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