bs-13118R [Primary Antibody]
Rabbit  Anti-EXOC2 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: EXOC2 Rabbit pAb

IR: Immunogen Range:31-130/924

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55770

Swiss Prot: Q96KP1

Source: KLH conjugated synthetic peptide derived from human EXOC2/SEC5:31-130/924 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The mammalian exocyst complex (sec 6/8) is a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Sec 5 is one of eight exocyst complex subunits: Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. Exocyst complex regulation depends upon Sec5 binding Ral in a GTP-dependent manner. Human Sec5 maps to chromosome 6p25.3.

Size: 200ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC2) Polyclonal Antibody, Unconjugated (bs-13118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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