Host: Rabbit
Target Protein: HBP1
IR: Immunogen Range:421-514/514
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 26959
Swiss Prot: O60381
Source: KLH conjugated synthetic peptide derived from human HBP1:421-514/514
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: HBP1 is a member of the HMG family of transcription factors, which are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, that mediates DNA binding. HBP-1 binds to the tumor suppressor proteins Rb and p130 and initiates cell cycle arrest.Terminal cell differentiation requires this initial cell cycle arrest followed by the coordinated expression of genes defined as tissue-specifc markers. Along with initiating the commitment to cell differentiation, the continued activity of HBP1 abrogates the expression of tissue-specific genes by associating with the MyoD proteins. In muscle cell differentiation, the MyoD family of transcription factors, which include Myf5, MyoD and myogenein, induce the expression of these cell-type specific proteins and contribute to the development of cell phenotypes. The progression of terminal differentiation is, therefore, dependent on both a decrease in HBP1 activity and the corresponding activation of MyoD-induced gene transcription.
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100-500, IF=1:100-500
Cross Reactive Species: Human,Mouse (predicted: Rat)
For research use only. Not intended for diagnostic or therapeutic use.