Host: Rabbit
Target Protein: phospho-eIF4E (Ser209)
IR: Immunogen Range:SG(p-S)TT
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1977
Swiss Prot: P06730
Source: KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209:SG(p-S)TT
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Size: 200ul
Concentration: 1mg/ml
Applications: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test, ELISA=1:5000-10000
Cross Reactive Species: Human,Mouse,Rat,Zebrafish (predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit)
For research use only. Not intended for diagnostic or therapeutic use.