bs-10296R [Primary Antibody]
Rabbit  Anti-Claudin 5 Polyclonal Antibody
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Host: Rabbit

Target Protein: Claudin 5

IR: Immunogen Range:29-81/218

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7122

Swiss Prot: O00501

Source: KLH conjugated synthetic peptide derived from human Claudin 5:29-81/218 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
ELISA(1:500-1000)
Flow-Cyt(3ug/Test)

Cross Reactive Species: Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Protein: lung(mouse) lysates at 45ug; Primary: Anti-Claudin 5 (bs-10296R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
ECL excitated the fluorescence;
Predicted band size :23 kD
Observed band size :23kD
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (bs-10296R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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