bs-10474R [Primary Antibody]
Rabbit  Anti-Serine racemase Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: Serine racemase Rabbit pAb

IR: Immunogen Range:1-100/430

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 63826

Swiss Prot: Q9GZT4

Source: KLH conjugated synthetic peptide derived from human Serine racemase:1-100/430 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Known to be prominent in bacteria, D amino acids were generally thought to be absent in mammals. D-serine has since been found in high levels in the mammalian brain and in various mammalian fluids. D-serine activates N-methyl-D-aspartate (NMDA) receptors--molecules with important roles in learning, brain growth and brain cell death. Serine racemase is the enzyme catalyzing the formation of D-serine from L-serine. Serine racemase is a member of the family of pyridoxal-5’ phosphate-dependent enzymes and is localized to glial cells in rat brain.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Serine racemase (bs-10474R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD
Sample: lovo (human)cell Lysate at 40 ug Primary: Anti- Serine racemase (bs-10474R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation:Anti-Serine racemase Polyclonal Antibody, Unconjugated(bs-10474R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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