Host: Rabbit

Target Protein: Histone H3 (Acetyl Lys9/14)

IR: Immunogen Range:AR(Acetyl-K)STGG(Acetyl-K)AP

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8350

Swiss Prot: P68431

Source: KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of Lys9/14:AR(Acetyl-K)STGG(Acetyl-K)AP 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.