bs-17183R [Primary Antibody]
Rabbit  Anti-ITPK1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: ITPK1 Rabbit pAb

IR: Immunogen Range:1-100/414

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3705

Swiss Prot: Q13572

Source: KLH conjugated synthetic peptide derived from human ITPK1:1-100/414 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Intracellular calcium concentrations are regulated by a family of inositol phosphates, which act as second messengers in the calcium cell signaling pathway. ITPK1 (inositol 1,3,4-triphosphate 5/6 kinase), also known as ITRPK1, is a 414 amino acid monomer that belongs to the ITPK1 family and exists as two alternatively spliced isoforms. Widely expressed, ITPK1 is found at highest levels in brain, followed by heart, skeletal muscle, kidney, pancreas, liver, placenta and lung. ITPK1 contains one ATP-grasp domain and has been found to phosphorylate various.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Zebrafish,Chicken,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- ITPK1 (bs-17183R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46kD Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ITPK1) Polyclonal Antibody, Unconjugated (bs-17183R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ITPK1) Polyclonal Antibody, Unconjugated (bs-17183R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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