Host: Rabbit

Target Protein: MSC/Musculin

IR: Immunogen Range:51-150/201

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 17681

Swiss Prot: O88940

Source: KLH conjugated synthetic peptide derived from mouse MSC/Musculin:51-150/201 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins, known to play an important role in the regulation of muscle cell development, includes Myo D, myogenin and musculin (also designated MyoR). Members of this group of transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E or musculin-E heterodimers bind avidly to consensus E box motifs, which are functionally important elements in the promoter regions of many muscle-specific terminal differentiation genes. Myo D complexes potently induce transcriptional activation, while musculin complexes bind adjacent to Myo D DNA-binding regions to represses Myo D activity, which then results in the delayed expression of muscle-specific genes. Musculin is highly expressed in undifferentiated and proliferating myoblasts in culture, and its expression is down regulated during myogenesis and at the onset of terminal differentiation.