Host: Rabbit

Target Protein: NPTII/Neomycin Phosphotransferase 2

IR: Immunogen Range:101-200/264

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: N/A

Swiss Prot: P00552

Source: KLH conjugated synthetic peptide derived from Klebsiella pneumoniae Neomycin Phosphotransferase 2:101-200/264 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.