bs-20152R [Primary Antibody]
Rabbit  Anti-DNCLI1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: DNCLI1 Rabbit pAb

IR: Immunogen Range:451-523/523

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51143

Swiss Prot: Q9Y6G9

Source: KLH conjugated synthetic peptide derived from human DNCLI1:451-523/523 

Purification: affinity purified by Protein A

Storage: Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: DNCLI1 (Cytoplasmic dynein 1 light intermediate chain 1) is a 523 amino acid protein that consists of at least 3 heavy chains, 2 intermediate chains and 8 light chains. DNCLI1 may play a role in binding Dynein heavy chain to chromosomes or membranous organelles and also may regulate Dynein enzymatic activity by associating with heavy chains of the Dynein head.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse (predicted: Rat,Chicken)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- DNCLI1 (bs-20152R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Tissue/cell: Human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DNCLI1 Polyclonal Antibody, Unconjugated(bs-20152R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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