bs-20452R [Primary Antibody]
Rabbit  Anti-DLX2 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: DLX2 Rabbit pAb

IR: Immunogen Range:51-150/328

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1746

Swiss Prot: Q07687

Source: KLH conjugated synthetic peptide derived from human DLX2:51-150/328 

Purification: affinity purified by Protein A

Storage: Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Dlx genes are a highly conserved family of six different (Dlx1–6) homeo box-containing genes that share homology with distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. Dlx genes are expressed in spatially and temporally restricted patterns in craniofacial primordia, basal telencephalon and diencephalon, and in distal regions of extending appendages, including the limb and the genital bud. The differential expression of Dlx influences patterning, morphogenesis and histogenesis in these tissues. The Dlx gene products can activate transcription and are localized primarily to the nucleus, although Dlx-5 can be found in the cytoplasm. Dlx proteins influence different stages of proper tissue development, including patterning of the orofacial skeleton (craniofacial ectomesenchyme) and differentiation of structures within and between teeth.

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse (predicted: Rat)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample:293T (Human)Cell Lysate at 40 ug Primary: Anti-DLX2(bs-20452R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 34kD Observed band size: 34kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DLX2) Polyclonal Antibody, Unconjugated (bs-20452R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DLX2) Polyclonal Antibody, Unconjugated (bs-20452R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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