Host: Rabbit
Target Protein: Raptor
IR: Immunogen Range:601-700/1335
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 57521
Swiss Prot: Q8N122
Source: KLH conjugated synthetic peptide derived from human Raptor:601-700/1335
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.
Size: 50ul
Concentration: 1mg/ml
Applications: IHC-P=1:100-500, ELISA=1:5000-10000
Cross Reactive Species: Human (predicted: Sheep,Horse,Cow,Pig,Chicken,Rat,Mouse)
For research use only. Not intended for diagnostic or therapeutic use.