bsm-33136M [Primary Antibody]
Mouse  Anti-HSP90 beta Monoclonal Antibody
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Host: Mouse

Target Protein: HSP90 beta

IR: Immunogen Range:

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3326

Swiss Prot: P08238

Source: KLH conjugated synthetic peptide derived from human HSP90 beta: 

Purification: affinity purified by Protein G

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-1000

Cross Reactive Species: Human,Mouse,Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: K562 (Human) Lysate at 30 ug Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD
Sample: Hela (Human) Lysate at 30 ug Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD
Sample: Hela KO HSP90 beta(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 4: K562 (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: U2os (Human) Cell Lysate at 30 ug Lane 7: A549 (Human) Cell Lysate at 30 ug Lane 8: HL60 (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: Testis (Mouse) Lysate at 40 ug Lane 11: Testis (Rat) Lysate at 40 ug Lane 12: Cerebrum (Mouse) Lysate at 40 ug Lane 13: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 86 kD
 
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