Host: Mouse
Target Protein: T7 tag
IR: Immunogen Range:MASMTGGQQMG
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: N/A
Swiss Prot: N/A
Source: KLH conjugated synthetic peptide T7-tag:MASMTGGQQMG
Purification: affinity purified by Protein G
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.
Size: 100ul
Concentration: 1mg/ml
Applications: WB=1:1000-5000, ELISA=1:5000-10000
Cross Reactive Species: Species independent
For research use only. Not intended for diagnostic or therapeutic use.