bs-23526R [Primary Antibody]
Rabbit  Anti-WEE1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: WEE1 Rabbit pAb

IR: Immunogen Range:151-250/646

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7465

Swiss Prot: P30291

Source: KLH conjugated synthetic peptide derived from human WEE1 :151-250/646 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Rat (predicted: Mouse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Heart (Rat) Lysate at 40 ug Primary: Anti-WEE1 (bs-23526R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 72 kD
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WEE1) Polyclonal Antibody, Unconjugated (bs-23526R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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