Host: Mouse
Target Protein: CDKN1B
IR: Immunogen Range:
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 1027
Swiss Prot: P46527
Source: Recombinant human CDKN1B:
Purification: affinity purified by Protein G
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
Size: 50ul
Concentration: 1mg/1ml
Applications: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:20-200, IF=1:20-200
Cross Reactive Species: (predicted: Human)
For research use only. Not intended for diagnostic or therapeutic use.