Host: Rabbit
Target Protein: A-RAF
IR: Immunogen Range:
Clonality:
Isotype: IgG
Entrez Gene: 369
Swiss Prot: P10398
Source: Recombinant human A-RAF protein (150-300aa):
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-200, ICC=1:50-200, IF=1:50-200
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.