Host: Rabbit
Target Protein: Smad3
IR: Immunogen Range:61-160/425
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4088
Swiss Prot: Q15796
Source: KLH conjugated synthetic peptide derived from human Smad3:61-160/425
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000
Cross Reactive Species: Human,Mouse (predicted: Dog,Pig,Cow,Horse,Sheep)
For research use only. Not intended for diagnostic or therapeutic use.