Host: Rabbit
Target Protein: LC3
IR: Immunogen Range:1-100/121
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 84557
Swiss Prot: Q9H492
Source: KLH conjugated synthetic peptide derived from human LC3:1-100/121
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000
Cross Reactive Species: Mouse (predicted: Human,Rat,Pig,Cow,Horse)
For research use only. Not intended for diagnostic or therapeutic use.