bsm-54196R [Primary Antibody]
Rabbit  Anti-BST2 Recombinant Rabbit mAb   Antibody
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Host: Rabbit

Target Protein: BST2 Recombinant Rabbit mAb

IR: Immunogen Range:

Clonality:

Isotype: IgG

Entrez Gene: 684

Swiss Prot: Q10589

Source: KLH conjugated synthetic peptide derived from human BST2: 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Bone marrow stromal cells act as regulators for B-cell growth and development through their surface molecules and cytokines. Bone marrow stromal antigen-2 (BST-2), also designated CD317 antigen, is a single- pass type II membrane protein. BST-2, which is expressed mainly on synovial cell lines and bone marrow stromal cell lines, is primarily expressed in liver, heart, placenta and lung tissues. BST-2 is thought to be involved in pre-B cell growth. It has been implicated in B cell activation in rheumatoid arthritis.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:300-500,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:100-500,Flow-Cyt=1:50

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Hela cell lysate Lane 2: Siha cell lysatee Primary: Anti-BST2 (bsm-54196R) at 1:500 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 18 kD Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (bsm-54196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (bsm-54196R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (bsm-54196R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (bsm-54196R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:Jurkat . Primary Antibody (green line): Rabbit Anti-BST2 antibody (bsm-54196R) Dilution: 1:50; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1:1000. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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