Host: Rabbit
Target Protein: Bcl-2
IR: Immunogen Range:21-120/236
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 596
Swiss Prot: P49950
Source: KLH conjugated synthetic peptide derived from human Bcl-2 :21-120/236
Purification: affinity purified by Protein A
Storage: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-300, ICC=1:100-500, ELISA=1:10000
Cross Reactive Species: Human,Mouse,Rat
For research use only. Not intended for diagnostic or therapeutic use.