bs-2715R [Primary Antibody]
Rabbit  Anti-THBS1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: THBS1 Rabbit pAb

IR: Immunogen Range:801-900/1170

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7057

Swiss Prot: P07996

Source: KLH conjugated synthetic peptide derived from human TSP-1:801-900/1170 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq].

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Zebrafish (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-THBS1 (bs-2715R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kD Observed band size: 129 kD
Paraformaldehyde-fixed, paraffin embedded (human myocardial tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THBS1) Polyclonal Antibody, Unconjugated (bs-2715R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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