Host: Rabbit
Target Protein: RUNX1
IR: Immunogen Range:101-200/453
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 861
Swiss Prot: Q01196
Source: KLH conjugated synthetic peptide derived from human RUNX1:101-200/453
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia。
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
Cross Reactive Species: Human,Mouse (predicted: Rat,Dog,Cow,Horse,Rabbit)
For research use only. Not intended for diagnostic or therapeutic use.