bs-3821R [Primary Antibody]
Rabbit  Anti-KDM1/LSD1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: KDM1/LSD1 Rabbit pAb

IR: Immunogen Range:101-200/852

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23028

Swiss Prot: O60341

Source: KLH conjugated synthetic peptide derived from human KDM1:101-200/852 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Lysine specific demethylase 1 (KDM1/LSD1) is a long-sought histone demethylase that specifically demethylates mono and di methyl histone H3 at K4 and K9. Thus KDM1 is a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: 293T (Human) Cell Lysate at 30 ug Lane 4: Jurkat (Human) Cell Lysate at 30 ug Primary: Anti-KDM1/LSD1 (bs-3821R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KDM1/LSD1) Polyclonal Antibody, Unconjugated (bs-3821R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; Frozen section; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KDM1 Polyclonal Antibody, Unconjugated(bs-3821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(black line):Hela. Primary Antibody (green line): Rabbit Anti-KDM1/LSD1 antibody (bs-3821R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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