bs-3882R [Primary Antibody]
Rabbit  Anti-GPX1 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: GPX1 Rabbit pAb

IR: Immunogen Range:51-150/201

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14775

Swiss Prot: P11352

Source: KLH conjugated synthetic peptide derived from mouse Glutathione Peroxidase 1:51-150/201 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene product belongs to the family of glutathione peroxidase, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test,ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat,Sheep (predicted: Pig,Cow,Dog,GuineaPig,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Spleen tissue lysates Lane 3: Mouse Kidney tissue lysates Lane 4: Rat Liver tissue lysates Lane 5: Rat Heart tissue lysates Lane 6: Rat Spleen tissue lysates Lane 7: Rat Kidney tissue lysates Primary: Anti-GPX1 (bs-3882R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- GPX1 (bs-3882R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD
Blank control:U937. Primary Antibody (green line): Rabbit Anti-GPX1 antibody (bs-3882R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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