bs-5044R [Primary Antibody]
Rabbit  Anti-COL4A3BP Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: COL4A3BP Rabbit pAb

IR: Immunogen Range:551-624/624

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10087

Swiss Prot: Q9Y5P4

Source: KLH conjugated synthetic peptide derived from human COL4A3BP:551-624/624 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: COL4A3BP is a kinase that specifically phosphorylates the N terminal region of the non collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Two transcripts exist for this gene.

Size: 200ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Rat (predicted: Human,Mouse,Rabbit,Cow)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COL4A3BP) Polyclonal Antibody, Unconjugated (bs-5044R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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