bs-6765R [Primary Antibody]
Rabbit  Anti-Perilipin A+B Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: Perilipin A+B Rabbit pAb

IR: Immunogen Range:Mixed peptides

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5346

Swiss Prot: O60240

Source: KLH conjugated synthetic peptide derived from human Perilipin A+B:Mixed peptides 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/Test

Cross Reactive Species: Human,Mouse (predicted: Sheep,Chicken,Dog)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-Perilipin A+B (bs-6765R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54-56kD Observed band size: 50kD
Sample: Stomach (Mouse) Lysate at 40 ug Primary: Anti-Perilipin A+B  (bs-6765R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54-56 kD Observed band size: 56 kD
This image was generously provided by James Fox, PhD. at the University of York. Immunostaining of perilipin-positive adipocytes (red) using Rabbit Anti-Perilipin A+B Polyclonal Antibody (bs-6765R) in bone marrow of IL-7cre Rosa26-EYFP mice with DAPI (blue) nuclear counterstain.
Blank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- Perilipin A+B antibody(bs-6765R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Blank control: MCF7(blue). Primary Antibody: Rabbit Anti-Perilipin A+B/AF647 Conjugated antibody (bs-6765R-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
 
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