bs-7686R [Primary Antibody]
Rabbit  Anti-GPR55 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: GPR55 Rabbit pAb

IR: Immunogen Range:141-240/319

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9290

Swiss Prot: Q9Y2T6

Source: KLH conjugated synthetic peptide derived from human GPR55:141-240/319 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Chicken,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: Human U87MG cell lysates Lane 2: Human U251 cell lysates Lane 3: Human U2os cell lysates Primary: Anti-GPR55 (bs-7686R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (bs-7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (bs-7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (bs-7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
 
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