Host: Rabbit
Target Protein: GM130
IR: Immunogen Range:851-1002/1002
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2801
Swiss Prot: Q08379
Source: KLH conjugated synthetic peptide derived from human GM130:851-1002/1002
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: The docking of transport vesicles to their target membrane is mediated by p115. GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115. Thus, p115-Giantin and p115-GM130 interactions might mediate independent membrane tethering events. Transport from the ER to the cis/medial Golgi compartments requires the action of p115, GM130 and Giantin via a sequential rather than a coordinate mechanism. Mitotic phosphorylation of GM130 at Serine 25 is mediated by Cdc2, prevents binding to p115 and is directly involved in mitotic Golgi fragmentation. GM130 is phosphorylated in prophase as the Golgi complex starts to break down, and remains phosphorylated in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble.
Size: 100ul
Concentration: 1mg/ml
Applications: IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Cross Reactive Species: Human,Rat (predicted: Mouse,Pig,Cow)
For research use only. Not intended for diagnostic or therapeutic use.