bs-1172R [Primary Antibody]
Rabbit  Anti-N-cadherin Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: N-cadherin Rabbit pAb

IR: Immunogen Range:701-800/905

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1000

Swiss Prot: P19022

Source: KLH conjugated synthetic peptide derived from human N-cadherin:701-800/905 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000

Cross Reactive Species: Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 3: U251 (Human) Cell Lysate at 30 ug Lane 4: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-N-cadherin (bs-1172R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-N-cadherin (bs-1172R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 105/125 kD
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Testis (Rat) Lysate at 40 ug Lane 4: Cerebellum (Rat) Lysate at 40 ug Lane 5: Panc-1 (Human) Cell Lysate at 30 ug Lane 6: A549 (Human) Cell Lysate at 30 ug Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 8: U251 (Human) Cell Lysate at 30 ug Primary: Anti-N-cadherin (bs-1172R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
 
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