bs-8347R [Primary Antibody]
Rabbit  Anti-UBE2I Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: UBE2I Rabbit pAb

IR: Immunogen Range:98-158/158

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7329

Swiss Prot: P63279

Source: KLH conjugated synthetic peptide derived from human UBE2I:98-158/158 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200

Cross Reactive Species: Mouse,Rat (predicted: Human)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-UBC9 (bs-8347R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 17 kD
Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-UBC9 (bs-8347R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 17 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBE2I/UBC9) Polyclonal Antibody, Unconjugated (bs-8347R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBE2I/UBC9) Polyclonal Antibody, Unconjugated (bs-8347R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 
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