bs-8394R [Primary Antibody]
Rabbit  Anti-Fbxw7 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: Fbxw7 Rabbit pAb

IR: Immunogen Range:501-600/707

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55294

Swiss Prot: Q969H0

Source: KLH conjugated synthetic peptide derived from human Fbxw7/CDC4:501-600/707 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The F-box protein family is characterized by an approximately 40 amino acid motif known as the F-box. F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. One family member, Cdc4, also known as AGO, FBW7, FBXW7, FBX30, SEL10, and FLJ11071, maps to human chromosome 4q31.3. Alternative splicing of this gene generates four transcript variants. In addition to an F-box, Cdc4 contains seven tandem WD40 repeats. Cdc4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Mutations of the Cdc4 gene are detected in ovarian and breast cancer cell lines, suggesting that the gene may be involved in the pathogenesis of human cancers.

Size: 50ul

Concentration: 1mg/ml

Applications: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=1ug/Test

Cross Reactive Species: Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbxw7) Polyclonal Antibody, Unconjugated (bs-8394R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbxw7) Polyclonal Antibody, Unconjugated (bs-8394R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Fbxw7/CDC4 Polyclonal Antibody, Unconjugated(bs-8394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:MCF-7. Primary Antibody (green line): Rabbit Anti-Fbxw7 antibody (bs-8394R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control（black line）:MCF-7. Primary Antibody (green line): Rabbit Anti-Fbxw7 antibody (bs-8394R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-Fbxw7 antibody (bs-8394R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
 
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