Host: Rabbit
Target Protein: ALAS1
IR: Immunogen Range:161-260/640
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 211
Swiss Prot: P13196
Source: KLH conjugated synthetic peptide derived from human ALAS1/ALAS-H:161-260/640
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.
Size: 50ul
Concentration: 1mg/ml
Applications: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, ELISA=1:5000-10000
Cross Reactive Species: Human,Mouse,Rat (predicted: Dog,Horse,Rabbit)
For research use only. Not intended for diagnostic or therapeutic use.