Host: Rabbit
Target Protein: CDKN1B/p27 KIP 1
IR: Immunogen Range:101-198/198
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1027
Swiss Prot: P46527
Source: KLH conjugated synthetic peptide derived from human P27 kip1:101-198/198
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
Size: 50ul
Concentration: 1mg/ml
Applications: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800, Flow-Cyt=1μg/Test , ELISA=1:5000-10000
Cross Reactive Species: Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Sheep)
For research use only. Not intended for diagnostic or therapeutic use.