bs-0485R [Primary Antibody]
Rabbit  Anti-EphA2 Rabbit pAb  Polyclonal Antibody
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Host: Rabbit

Target Protein: EphA2 Rabbit pAb

IR: Immunogen Range:901-976/976

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1969

Swiss Prot: P29317

Source: KLH conjugated synthetic peptide derived from human EphA2:901-976/976 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010].

Size: 100ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500

Cross Reactive Species: Human,Mouse,Rat (predicted: Cow,Dog,Monkey)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Human colon carcinoma lysates Lysate at 30 ug Intestine (Mouse) Lysate at 30 ug Primary: Anti- EphA2/Eph receptor A2 (bs-0485R) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution Predicted band size : 105kD Observed band size : 105kD, 52/55kD
Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-EphA2 (bs-0485R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kD Observed band size: 115 kD
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EphA2/Eph receptor A2 Polyclonal Antibody, Unconjugated(bs-0485R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
 
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