Host: Rabbit
Target Protein: phospho-CDKN1A/p21 (Thr57)
IR: Immunogen Range:TE(p-T)PL
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1026
Swiss Prot: P38936
Source: KLH conjugated Synthesised phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr57:TE(p-T)PL
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Size: 200ul
Concentration: 1mg/ml
Applications: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
Cross Reactive Species: Human,Mouse,Rat
For research use only. Not intended for diagnostic or therapeutic use.