bs-2007R [Primary Antibody]
Rabbit  Anti-PCNA  Polyclonal Antibody
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Host: Rabbit

Target Protein: PCNA

IR: Immunogen Range:201-261/261

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5111

Swiss Prot: P12004

Source: KLH conjugated synthetic peptide derived from human PCNA:201-261/261 

Purification: affinity purified by Protein A

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background: Proliferation Marker

Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure。

Size: 200ul

Concentration: 1mg/ml

Applications: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000

Cross Reactive Species: Human,Mouse,Rat (predicted: Chicken,Dog,Cow,Rabbit)

For research use only. Not intended for diagnostic or therapeutic use.

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