bs-4185R [Primary Antibody]
Rabbit  Anti-RelB Rabbit pAb  Polyclonal Antibody
www.bioss.com.cn
sales@bioss.com.cn
techsupport@bioss.com.cn
400-901-9800
DATASHEET

Host: Rabbit

Target Protein: RelB Rabbit pAb

IR: Immunogen Range:161-260/579

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5971

Swiss Prot: Q01201

Source: KLH conjugated synthetic peptide derived from human RelB:161-260/579 

Purification: affinity purified by Protein A

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: The NFKB complex consists of NFKB1 or NFKB2 bound to REL, RELA, or RELB. The NFKB complex is inhibited by I kappa B proteins (NFKBIA, or NFKBIB), which inactivate NF kappa B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I kappa B proteins by kinases (IKBKA, or IKBKB,) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF kappa B complex.

Size: 50ul

Concentration: 1mg/ml

Applications: WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:20-100

Cross Reactive Species: Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES
Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-RelB (bs-4185R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti-RelB (bs-4185R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RelB) Polyclonal Antibody, Unconjugated (bs-4185R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: mouse blood primary antibody: Anti-RelB/PE-Cy5(bs-4185R-PE-Cy5). concentration:1:20, 4℃ incubate 30min.
 
PRODUCT SPECIFIC PUBLICATIONS