Host: Rabbit
Target Protein: Cullin 3
IR: Immunogen Range:411-510/768
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8452
Swiss Prot: Q13618
Source: KLH conjugated synthetic peptide derived from human Cullin 3:411-510/768
Purification: affinity purified by Protein A
Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background: Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
Size: 50ul
Concentration: 1mg/ml
Applications: IF=1:100-500, ELISA=1:5000-10000
Cross Reactive Species: Human (predicted: Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
For research use only. Not intended for diagnostic or therapeutic use.