| 产品编号 | bsm-61144R |
| 英文名称 | SUMO1 Recombinant Rabbit mAb |
| 中文名称 | 类泛素蛋白1重组兔单抗 |
| 别 名 | DAP-1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO1; UBL1. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 5F3 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12 kDa |
| 检测分子量 | 12 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Sumo 1: 70-101 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SUMO1 Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SWISS: P63165 Gene ID: 7341 合成与降解（Synthesis and Degradation） SUMO化能够使蛋白质更加稳定,SUMO的生化反应途径与泛素相似,但不像泛素那样诱导底物蛋白降解.进而调节许多关键的细胞活动. SUMO(small ubiquitin-related modifier)是泛素(ubiqutin)类蛋白家族的重要成员之一. |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with SUMO1 monoclonal antibody, unconjugated (bsm-61144R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HeLa(treated with MG-132 (10μM, 1hr) ）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SUMO1) monoclonal Antibody, unconjugated (bsm-61144R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
