| 产品编号 | bsm-61373R |
| 英文名称 | Integrin alpha 4 Recombinant Rabbit mAb |
| 中文名称 | 整合素α4（CD49d）重组兔单抗 |
| 别 名 | CD49d; ITA4; Integrin alpha-4 precursor; Integrin alpha-IV; VLA4; ITGA4. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 6F8 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 115 kDa |
| 检测分子量 | 150 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Integrin alpha 4: 1000-1032 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. SWISS: P13612 Gene ID: 3676 整合素-α-4型 Integrin α4 (CD49d)整合素-α是介导细胞与细胞外基质粘附作用的主要因子。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Integrin alpha 4 monoclonal antibody, unconjugated (bsm-61373R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
